Determination of DNA Damage in Floriculturists Exposed to  Mixtures
of Pesticides

Castillo-Cadena, J.; Tenorio-Vieyra, L. E.; Quintana-Carabia, A. I.; García-Fabila, M. M.; Juan, E. Ramírez-San; Madrigal-Bujaidar, E.

doi:https://doi.org/10.1155/JBB/2006/97896

BioMed Research International

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Research Article | Open Access

Volume 2006 | Article ID 097896 | https://doi.org/10.1155/JBB/2006/97896

Determination of DNA Damage in Floriculturists Exposed to Mixtures of Pesticides

J. Castillo-Cadena,^1,2L. E. Tenorio-Vieyra,²A. I. Quintana-Carabia,²M. M. García-Fabila,²E. Ramírez-San Juan,¹and E. Madrigal-Bujaidar^1,3

Received05 Apr 2005

Revised18 Oct 2005

Accepted19 Oct 2005

Published21 Feb 2006

Abstract

The aim of the study was to determine possible DNA damage in floriculturists chronically exposed to pesticides. Leukocytes from 52 workers, 46 environmentally exposed, and 38 control individuals were evaluated with the comet assay. Serum from all individuals was also analyzed for pesticides using gas chromatography coupled to mass spectrometry. A statistically significant difference in DNA fragmentation in the pesticide exposed group compared to the other two groups (P <.001) was found. No differences between environmentally exposed and control individuals were detected. The statistical analysis showed no significant correlation between DNA damage and sex, age, drinking or smoking habits, as well as years of exposure. One or more pesticides were detected in 50% of the floriculturists, while in the rest of the individuals, a chemical related with the preparation of pesticides, such as additives, plasticizers, or solvents, was found. Our study shows that chronic exposure to pesticides produces DNA damage in floriculturists. It also suggests that this type of monitoring could be valuable in recommending preventive measures.

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Copyright © 2006 J. Castillo-Cadena et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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