BioMed Research International

BioMed Research International / 2007 / Article
Special Issue

Biomedical Applications of Colloidal Nanocrystals

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Research Article | Open Access

Volume 2007 |Article ID 26796 | 9 pages | https://doi.org/10.1155/2007/26796

Gel Electrophoresis of Gold-DNA Nanoconjugates

Academic Editor: Marek Osinski
Received20 Jul 2007
Accepted13 Dec 2007
Published31 Mar 2008

Abstract

Gold-DNA conjugates were investigated in detail by a comprehensive gel electrophoresis study based on 1200 gels. A controlled number of single-stranded DNA of different length was attached specifically via thiol-Au bonds to phosphine-stabilized colloidal gold nanoparticles. Alternatively, the surface of the gold particles was saturated with single stranded DNA of different length either specifically via thiol-Au bonds or by nonspecific adsorption. From the experimentally determined electrophoretic mobilities, estimates for the effective diameters of the gold-DNA conjugates were derived by applying two different data treatment approaches. The first method is based on making a calibration curve for the relation between effective diameters and mobilities with gold nanoparticles of known diameter. The second method is based on Ferguson analysis which uses gold nanoparticles of known diameter as reference database. Our study shows that effective diameters derived from gel electrophoresis measurements are affected with a high error bar as the determined values strongly depend on the method of evaluation, though relative changes in size upon binding of molecules can be detected with high precision. Furthermore, in this study, the specific attachment of DNA via gold-thiol bonds to Au nanoparticles is compared to nonspecific adsorption of DNA. Also, the maximum number of DNA molecules that can be bound per particle was determined.

Supplementary Materials

The Supplemental material contains detailed information of all experiments and protocols, tables of all data, and the DNA sequences used for this work.

  1. Supplementary text

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Copyright © 2007 T. Pellegrino et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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