BioMed Research International

BioMed Research International / 2007 / Article
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Biomedical Applications of Colloidal Nanocrystals

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Volume 2007 |Article ID 068963 | https://doi.org/10.1155/2007/68963

Camilla Luccardini, Aleksey Yakovlev, Stéphane Gaillard, Marcel van ‘t Hoff, Alicia Piera Alberola, Jean-Maurice Mallet, Wolfgang J. Parak, Anne Feltz, Martin Oheim, "Getting Across the Plasma Membrane and Beyond: Intracellular Uses of Colloidal Semiconductor Nanocrystals", BioMed Research International, vol. 2007, Article ID 068963, 9 pages, 2007. https://doi.org/10.1155/2007/68963

Getting Across the Plasma Membrane and Beyond: Intracellular Uses of Colloidal Semiconductor Nanocrystals

Academic Editor: Marek Osinski
Received13 Apr 2007
Accepted12 Oct 2007
Published15 Nov 2007

Abstract

Semiconductor nanocrystals (NCs) are increasingly being used as photoluminescen markers in biological imaging. Their brightness, large Stokes shift, and high photostability compared to organic fluorophores permit the exploration of biological phenomena at the single-molecule scale with superior temporal resolution and spatial precision. NCs have predominantly been used as extracellular markers for tagging and tracking membrane proteins. Successful internalization and intracellular labelling with NCs have been demonstrated for both fixed immunolabelled and live cells. However, the precise localization and subcellular compartment labelled are less clear. Generally, live cell studies are limited by the requirement of fairly invasive protocols for loading NCs and the relatively large size of NCs compared to the cellular machinery, along with the subsequent sequestration of NCs in endosomal/lysosomal compartments. For long-period observation the potential cytotoxicity of cytoplasmically loaded NCs must be evaluated. This review focuses on the challenges of intracellular uses of NCs.

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Copyright © 2007 Camilla Luccardini et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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