Abstract
A method of fluorescent nanoparticle-based indirect immunofluorescence microscopy
(FNP-IIFM) was developed for the rapid detection of Mycobacterium tuberculosis.
An anti-Mycobacterium tuberculosis antibody was used as primary antibody to recognize
Mycobacterium tuberculosis, and then an antibody binding protein (Protein A) labeled with
Tris(2,2-bipyridyl)dichlororuthenium(II) hexahydrate (RuBpy)-doped silica nanoparticles was
used to generate fluorescent signal for microscopic examination. Prior to the detection, Protein A
was immobilized on RuBpy-doped silica nanoparticles with a coverage of