Figure 1: Transient, short-term GFP fluorescence expression by the rat hepatic ASC strain, Lig-8, is associated with cell death. Cell strain, Lig-8, was transfected with the pEGFP-N3 expression vector. Shown are images of 5 independent colonies transiently expressing GFP fluorescence at 72–96 hours posttransfection. Shown are the phases (a)–(e), GFP-fluorescence (f)–(j), propidium iodide (PI) fluorescence (k)–(o), and merged GFP and PI fluorescence (p)–(t) images. Arrows highlight specific examples of cells double-positive for GFP and PI fluorescence in Colony no. 12. Scale bar is equivalent to 100 μm.