CFP and YFP, but Not GFP, Provide Stable Fluorescent Marking of Rat Hepatic Adult Stem Cells

Figure 3

Fluorescent protein (CFP or YFP)-expressing colonies from rat hepatic ASCs. Rat hepatic ASC strain, Lig-8, was stably transfected with either a CFP or YFP expression vector. Shown are colonies with approximately 20–75% of the cells expressing (B2) and colonies with essentially 100% of the cells expressing (B3). Shown are the phases (a)–(d), fluorescent (e)–(h), and merged (i)–(l) images of CFP- and YFP-expressing colonies. Scale bar is equivalent to 100 μm.