A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter

<table>PCR product of HBs gene: M: Markers; 1, 2, and 3: the samples from three single
embryos with green fluorescence showing HBs DNA positive bands, respectively; 4, 5, and 6:
the samples from three single embryos without green fluorescence showing negative results
for HBs DNA; 7: the positive control; 8: the negative control.</table>

BioMed Research International

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Figure 5

Figure 5: A Sensitive and Rapid Assay for Investigating Vertical Transmission of Hepatitis B Virus via Male Germ Line Using EGFP Vector as Reporter 