Simple Detection of Large InDeLS by DHPLC: The ACE Gene as a Model
Figure 1
Observed chromatograms patterns in the DHPLC system
at 50°C after submitting the same samples to the two conditions. (a) ACE I
sequence and chromatogram. (b) ACE D sequence and chromatogram. The lines in the upper part of both chromatograms show I/I genotype, the line in the middle is the D/D genotype, and the line below is the I/D genotype.
The homozygotes show a peak when their sequences are analyzed, and the
heterozygotes show a peak in both analyses.
