Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT
in Escherichia coli
Figure 1
Schematic
representation of the cloning steps of VEGI-CTT fragment into the
expression vector pET30a(+). (a) The vector Psw200 containing
full-length VEGI gene was used to amplify recombinant cDNA of human
VEGI-CTT for 483 bp by PCR. The recombinant cDNA of human VEGI-CTT
was cloned into pGEM-T, and produced pGEM-T-VEGI-CTT. (b) pET30a(+)
vector with His-tag upstream of the multiple cloning site that
contains XbaI and XhoI.
(c) pET30a(+) carrying XbaI-XhoI fragment of VEGI-CTT
to produce pET30a(+)-VEGI-CTT. The resulting fusion gene contains
internal His-tag and VEGI-CTT.
