Preparation and Characterization of a Novel Chimeric Protein VEGI-CTT
in Escherichia coli
Figure 2
Construction of prokaryotic expression vector pET-VEGI-CTT. (a)
DNA fragments of VEGI-CTT were amplified by PCR. Lane 1: 100 bp
markers. Lane 2: VEGI-CTT fragment of 486 bp. (b) Restriction enzyme
analysis of the prokaryotic expression vector pET-VEGI-CTT. Lane 1: 100 bp markers; lane 2: pET-VEGI-CTT digested by Xba I and Xho
I.