BioMed Research International / 2008 / Article / Fig 5

Research Article

Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Figure 5

The effect of the recombinant protein production by oleic acid-inducible promoter in recombinant E. coli W3110. The cells are induced by exchanging medium supplemented with glucose into the one with oleic acid at the OD600 of 0.7 (a) or 1.2 (b). For the control, cells harboring the plasmid containing tac promoter were added with 1 mM IPTG at the same values of OD600 in the defined medium supplemented with glucose. After induction by IPTG or oleic acid, cells were further cultured for 5, 10, 20 hours, and harvested for 12% (w/v) SDS-PAGE. The arrows indicate the green fluorescent protein (GFP; 26.9 kDa). Size markers (in kDa) are also indicated.

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