Proteome-Level Responses of Escherichia coli to Long-Chain Fatty Acids and Use of Fatty Acid Inducible Promoter in Protein Production

Figure 5

The effect of the recombinant protein production by oleic acid-inducible promoter in recombinant E. coli W3110. The cells are induced by exchanging medium supplemented with glucose into the one with oleic acid at the OD₆₀₀ of 0.7 (a) or 1.2 (b). For the control, cells harboring the plasmid containing tac promoter were added with 1 mM IPTG at the same values of OD₆₀₀ in the defined medium supplemented with glucose. After induction by IPTG or oleic acid, cells were further cultured for 5, 10, 20 hours, and harvested for 12% (w/v) SDS-PAGE. The arrows indicate the green fluorescent protein (GFP; 26.9 kDa). Size markers (in kDa) are also indicated.

(a)

(b)