BioMed Research International / 2008 / Article / Fig 2

Research Article

Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer

Figure 2

Construction of the concatemer Tα1 gene. A T-vector containing the Tα1 gene was digested with BamH I/Xho I and Bgl II/Xho I. When digested with Bgl II and BamH I, the 2 fragments have identical termini. The two fragments containing the Tα1 gene were ligated with T4 DNA Ligase. A plasmid containing double Tα1 genes was constructed. When the 2 fragments were catalyzed with T4 DNA Ligase, the new sequence formed was GGATCT, and this new site could not be digested by BamH I or Bgl II. Therefore, the new 2 concatemer gene could not be destroyed when the 4 concatemer gene was constructed. Thus, the plasmid containing 4 , 6 Tα1 genes could also be constructed.
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