Expression and Hydroxylamine Cleavage of Thymosin Alpha 1 Concatemer

Figure 2

Construction of the concatemer Tα1 gene. A T-vector containing the Tα1 gene was digested with BamH I/Xho I and Bgl II/Xho I. When digested with Bgl II and BamH I, the 2 fragments have identical termini. The two fragments containing the Tα1 gene were ligated with T4 DNA Ligase. A plasmid containing double Tα1 genes was constructed. When the 2 fragments were catalyzed with T4 DNA Ligase, the new sequence formed was GGATCT, and this new site could not be digested by BamH I or Bgl II. Therefore, the new 2× concatemer gene could not be destroyed when the 4× concatemer gene was constructed. Thus, the plasmid containing 4×, 6×Tα1 genes could also be constructed.