Characterization for Binding Complex Formation with Site-Directly Immobilized Antibodies Enhancing Detection Capability of Cardiac Troponin I
Figure 2
Affinity purification of the digested fragments of IgG type antibody on a protein G column and their characterization. Elution fractions from the column were analyzed for total protein by means of a Bradford assay (a). The fractions in each peak were pooled, which were then identified by molecular size via SDS-PAGE under non-reducing conditions (inset, (a). The reactivities of each pooled fraction were subsequently confirmed by solid-phase immunoassays with cTnI as the antigen immobilized on the solid matrix (b)
