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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 157531, 8 pages
http://dx.doi.org/10.1155/2009/157531
Research Article

Isolation of Osteosarcoma-Associated Human Antibodies from a Combinatorial Fab Phage Display Library

1Institut Gustave Roussy, UPRES EA 3535 PR2. 39, Rue Camille Desmoulins, 94805 Villejuif, France
2Departamento de Bioquímica e Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, ICB II-Campus II Samambaia, 74001-970 Goiania, GO, Brazil
3Departamento de Biologia Celular, Instituto de Ciências Biológicas, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Asa Norte, 70910900 Brasilia, DF, Brazil
4Laboratório de Imunologia Molecular, Universidade de Brasília, Campus Universitário Darcy Ribeiro, Sala ASS 128, ICC Sul, Asa Norte, 70910-900 Brasilia, DF, Brazil

Received 8 February 2009; Revised 29 June 2009; Accepted 9 September 2009

Academic Editor: Anthony L. DeVico

Copyright © 2009 Carmela Dantas-Barbosa et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. H. D. Dorfman and B. Czerniak, “Bone cancers,” Cancer, vol. 75, no. 1, pp. 203–210, 1995. View at Google Scholar · View at Scopus
  2. D. C. Dahlin and K. K. Unni, “Osteosarcoma of bone and its important recognizable varieties,” The American Journal of Surgical Pathology, vol. 1, no. 1, pp. 61–72, 1977. View at Google Scholar · View at Scopus
  3. G. Saeter, “Osteosarcoma: ESMO clinical recommendations for diagnosis, treatment and follow-up,” Annals of Oncology, vol. 18, supplement 2, pp. ii77–ii78, 2007. View at Publisher · View at Google Scholar · View at Scopus
  4. P. A. Meyers and R. Gorlick, “Osteosarcoma,” Pediatric Clinics of North America, vol. 44, no. 4, pp. 973–989, 1997. View at Publisher · View at Google Scholar · View at Scopus
  5. Ž. Pohar-Marinšek, “Difficulties in diagnosing small round cell tumours of childhood from fine needle aspiration cytology samples,” Cytopathology, vol. 19, no. 2, pp. 67–79, 2008. View at Publisher · View at Google Scholar · View at Scopus
  6. S. Salas, C. Bartoli, J.-L. Deville et al., “Ezrin and alpha-smooth muscle actin are immunohistochemical prognostic markers in conventional osteosarcomas,” Virchows Archiv, vol. 451, no. 6, pp. 999–1007, 2007. View at Publisher · View at Google Scholar · View at Scopus
  7. S. Salas, G. De Pinieux, A. Gomez-Brouchet et al., “Ezrin immunohistochemical expression in cartilaginous tumours: a useful tool for differential diagnosis between chondroblastic osteosarcoma and chondrosarcoma,” Virchows Archiv, vol. 454, no. 1, pp. 81–87, 2009. View at Publisher · View at Google Scholar · View at Scopus
  8. R. Brissette, J. K. A. Prendergast, and N. I. Goldstein, “Identification of cancer targets and therapeutics using phage display,” Current Opinion in Drug Discovery and Development, vol. 9, no. 3, pp. 363–369, 2006. View at Google Scholar · View at Scopus
  9. R. J. Giordano, M. Cardó-Vila, J. Lahdenranta, R. Pasqualini, and W. Arap, “Biopanning and rapid analysis of selective interactive ligands,” Nature Medicine, vol. 7, no. 11, pp. 1249–1253, 2001. View at Publisher · View at Google Scholar · View at Scopus
  10. C. Dantas-Barbosa, M. M. Brígido, and A. Q. Maranhão, “Construction of a human Fab phage display library from antibody repertoires of osteosarcoma patients,” Genetics and Molecular Research, vol. 4, no. 2, pp. 126–140, 2005. View at Google Scholar · View at Scopus
  11. C. Rader, R. P. Steinberger, and C. F. Barbas III, “Selection from antibody libraries,” in Phage Display : A Laboratory Manual, C. F. Barbas III, D. R. Burton, J. K. Scott, and G. J. Silverman, Eds., pp. 10.2–10.20, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA, 1st edition, 2000. View at Google Scholar
  12. J. Sambrook and D. W. Russel, Molecular Cloning: A Laboratory Manual, Cold Spring Harbor Laboratory Press, New York, NY, USA, 3rd edition, 2001.
  13. T. A. Hall, “BioEdit: a user-friendly biological sequence alignment editor and analysis,” Nucleic Acids Symposium Series, vol. 41, pp. 95–98, 1999. View at Google Scholar
  14. B. R. Williams and J. Sharon, “Polyclonal anti-colorectal cancer Fab phage display library selected in one round using density gradient centrifugation to separate antigen-bound and free phage,” Immunology Letters, vol. 81, no. 2, pp. 141–148, 2002. View at Publisher · View at Google Scholar · View at Scopus
  15. I. M. Tomlinson, J. P. L. Cox, E. Gherardi, A. M. Lesk, and C. Chothia, “The structural repertoire of the human V(κ) domain,” The EMBO Journal, vol. 14, no. 18, pp. 4628–4638, 1995. View at Google Scholar · View at Scopus
  16. S. Mao, C. Gao, C.-H. L. Lo, P. Wirsching, C.-H. Wong, and K. D. Janda, “Phage-display library selection of high-affinity human single-chain antibodies to tumor-associated carbohydrate antigens sialyl Lewisx and Lewisx,” Proceedings of the National Academy of Sciences of the United States of America, vol. 96, no. 12, pp. 6953–6958, 1999. View at Google Scholar · View at Scopus
  17. B. M. Edwards, S. H. Main, K. L. Cantone, S. D. Smith, A. Warford, and T. J. Vaughan, “Isolation and tissue profiles of a large panel of phage antibodies binding to the human adipocyte cell surface,” Journal of Immunological Methods, vol. 245, no. 1-2, pp. 67–78, 2000. View at Publisher · View at Google Scholar · View at Scopus
  18. P. U. Ardelt, C. G. Wood, L. Chen et al., “Targeting urotheliumml: ex vivo assay standardization and selection of internalizing ligands,” Journal of Urology, vol. 169, no. 4, pp. 1535–1540, 2003. View at Publisher · View at Google Scholar · View at Scopus
  19. W.-D. Jia, H.-C. Sun, J.-B. Zhang et al., “A novel peptide that selectively binds highly metastatic hepatocellular carcinoma cell surface is related to invasion and metastasis,” Cancer Letters, vol. 247, no. 2, pp. 234–242, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. U. B. Rasmussen, V. Schreiber, H. Schultz, F. Mischler, and K. Schughart, “Tumor cell-targeting by phage-displayed peptides,” Cancer Gene Therapy, vol. 9, no. 7, pp. 606–612, 2002. View at Publisher · View at Google Scholar · View at Scopus
  21. T. Oyama, K. F. Sykes, K. N. Samli, J. D. Minna, S. A. Johnston, and K. C. Brown, “Isolation of lung tumor specific peptides from a random peptide library: generation of diagnostic and cell-targeting reagents,” Cancer Letters, vol. 202, no. 2, pp. 219–230, 2003. View at Publisher · View at Google Scholar · View at Scopus
  22. R. Mutuberria, S. Satijn, A. Huijbers et al., “Isolation of human antibodies to tumor-associated endothelial cell markers by in vitro human endothelial cell selection with phage display libraries,” Journal of Immunological Methods, vol. 287, no. 1-2, pp. 31–47, 2004. View at Publisher · View at Google Scholar · View at Scopus
  23. H. Wu, J. D. Pancook, G. Beuerlein et al., “Cloning, isolation and characterization of human tumor in situ monoclonal antibodies,” Cancer Immunology, Immunotherapy, vol. 51, no. 2, pp. 79–90, 2002. View at Publisher · View at Google Scholar · View at Scopus
  24. Y. Shen, X. Yang, N. Dong, X. Xie, X. Bai, and Y. Shi, “Generation and selection of immunized Fab phage display library against human B cell lymphoma,” Cell Research, vol. 17, no. 7, pp. 650–660, 2007. View at Publisher · View at Google Scholar · View at Scopus
  25. D. N. T. Aryee, M. Kreppel, R. Bachmaier et al., “Single-chain antibodies to the EWS NH2 terminus structurally discriminate between intact and chimeric EWS in Ewing's sarcoma and interfere with the transcriptional activity of EWS in vivo,” Cancer Research, vol. 66, no. 20, pp. 9862–9869, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. X. Cai and A. Garen, “Anti-melanoma antibodies from melanoma patients immunized with genetically modified autologous tumor cells: selection of specific antibodies from single-chain Fv fusion phage libraries,” Proceedings of the National Academy of Sciences of the United States of America, vol. 92, no. 14, pp. 6537–6541, 1995. View at Publisher · View at Google Scholar · View at Scopus
  27. S. Pereira, H. Maruyama, D. Siegel et al., “A model system for detection and isolation of a tumor cell surface antigen using antibody phage display,” Journal of Immunological Methods, vol. 203, no. 1, pp. 11–24, 1997. View at Publisher · View at Google Scholar · View at Scopus