Ferromagnetic Levan Composite: An Affinity Matrix to Purify Lectin
Figure 3
Affinity binding of Cramoll 1,4 on FMZAG-12L. An amount of 1.5 mg of Cramoll was incubated with the magnetic particles and eluted with buffer and glucose afterwards (arrow). (a) Fractions (1 mL) were collected and their absorbance at 280 nm established (a). Each bar represents the mean SD of four experiments using the same FMZAG-12L particles. (b) Electrophoresis to native and basic protein (g) from the 14th and 15th fractions, stained with Amido Black.
