Engineering and Directed Evolution of a Ca2+ Binding Site A-Deficient AprE Mutant Reveal an Essential Contribution of the Loop Leu75–Leu82 to Enzyme Activity
Figure 1
Extracellular proteolytic activity of strains B. subtilis PERM200 (A), PERM222 (B), PERM505 (C), and PERM658 (D). 10 L aliquots were taken from cell cultures (O. of 0.5) and deposited on LB agar plates containing 2% (w/v) skimmed milk. The plates were incubated overnight at 37°C to allow the developing of halos of hydrolysis.
