Research Article

Caenorhabditis elegans F09E10.3 Encodes a Putative 3-Oxoacyl-Thioester Reductase of Mitochondrial Type 2 Fatty Acid Synthase FASII that Is Functional in Yeast

Figure 2

Phenotype rescue of a yeast oar1Δ strain expressing F09E10.3. Mutant S. cerevisiae BY4741oar1Δ cells producing native mitochondrial Oar1p (positive control), peroxisomal catalase A Cta1p (negative control), or the nematode mitochondrial protein F09E10.3 (tester strain), were grown on SD-Ura medium selecting for plasmid presence, and following tenfold serial dilution (triangle) were spotted onto the indicated solid media. Respiration competence was examined by applying 2,3,5-triphenyltetrazolium chloride (TTC) as an overlay to the SD-Ura medium.
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