BioMed Research International / 2009 / Article / Fig 2

Research Article

Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells

Figure 2

Expression of hCT mRNA and protein in NIH3T3 cells. (a). Ig -hCT mRNA expression in NIH3T3 cells by RT-PCR. Lane M: marker; Lane 1: RT-PCR products from pCDNA3.0-Ig -hCT-transfected NIH3T3 cells; Lane 2: RT-PCR product from pCDNA3.0-transfected cells (negative control); Lane 3: RT-PCR product from nontransfected cells. (b), Immunohistological staining of hCT protein in pCDNA3.0-Ig -hCT-transfected (b1), nontransfected (b2) and pCDNA3.0-transfected NIH3T3 cells (b3). All magnification 200. (c). Western blotting showing hCT protein was detected in pCDNA3.0-Igk-hCT-transfected cells (lanes 1–4), but not in pcDNA3.0 transfected cells (lane 5).
241390.fig.002a
(a)
241390.fig.002b
(b)
241390.fig.002c
(c)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.