Construction of a Recombinant Eukaryotic Expression Plasmid Containing Human Calcitonin Gene and Its Expression in NIH3T3 Cells
Primarily isolated osteoclasts cocultured with nontransfected (a) and pCDNA3.0-transfected (b), pCDNA3.0-Ig-hCT-transfected (c) NIH3T3 cells and nontransfected NIH3T3 cells plus sCT (d). TRAP staining shows that primarily isolated osteoclasts are multinucleate and spreading cells, which become crumpled when cocultured with pCDNA3.0-Ig-hCT-transfected.
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