241390.fig.003a
(a)
241390.fig.003b
(b)
241390.fig.003c
(c)
241390.fig.003d
(d)
Figure 3: Primarily isolated osteoclasts cocultured with nontransfected (a) and pCDNA3.0-transfected (b), pCDNA3.0-Ig -hCT-transfected (c) NIH3T3 cells and nontransfected NIH3T3 cells plus sCT (d). TRAP staining shows that primarily isolated osteoclasts are multinucleate and spreading cells, which become crumpled when cocultured with pCDNA3.0-Ig -hCT-transfected.