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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 274740, 6 pages
Research Article

Anxiolytic and Anticonvulsant Effects on Mice of Flavonoids, Linalool, and 𝛼 -Tocopherol Presents in the Extract of Leaves of Cissus sicyoides L. (Vitaceae)

1Evaluation Laboratory of Psychobioactive and Toxicology, Department of Antibiotics, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil
2Department of Odontology, Federal University of Pernambuco, 50670-901 Recife, PE, Brazil

Received 6 September 2008; Revised 7 November 2008; Accepted 15 December 2008

Academic Editor: Omar Benzakour

Copyright © 2009 Edvaldo Rodrigues de Almeida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The aim of the present study is to demonstrate the anxiolytic and anticonvulsant effects of a hydroalcoholic extract obtained from the aerial parts of Cissus sicyoides L. (CS) (Vitaceae) on male and female mice using several behavioral assays. Groups of males and females treated via intraperitoneal (IP) with doses of 300, 600, and 1000 mg/kg of the extract showed significant action in the elevated plus-maze (EPM), time spent in the open arms, and number of entries in the open arms. The board-hole test also showed a significant increase in the time spent in head-dipping and in marble-burying test of the number of marbles buried. The same treatment increased the duration of sleeping time induced by sodium pentobarbital and also showed a significant increase in protection against pentylenotetrazole-induced convulsions. These results indicate an anxiolytic and anticonvulsant-like action from C. sicyoides L. extract on mice, probably due to the action of flavonoid(s), Linalool, and 𝛼 -tocopherol present in the C. sicyoides leaves.