275287.fig.002a
(a)
275287.fig.002b
(b)
Figure 2: Recovery of B. breve (closed circles) and B. breve (open circles) from female BALB/c mice over 32 days of analysis. (a) Faeces for bacteriological analysis were obtained from five mice in each treatment group and viable counts of B. breve derivatives were determined, adapted from Sheehan et al. [9]. (b) Listerial infection in the livers and spleens of BALB/c mice. Animals were fed 109 CFU of either B. breve or the betL negative control strain B. breve for three consecutive days. A control group was fed PBS. On the fourth day, all animals were infected with 1011 CFU luminescent L. monocytogenes EGD-e. Three days postlisterial infection the animals were sacrificed and the extent of listerial infection of the liver determined by measuring light intensity using an IVIS 100 system.