Nitric Oxide Production by the Human Intestinal Microbiota by Dissimilatory Nitrate Reduction to Ammonium

Figure 4

NO-N concentrations produced by the microbiota of the 3 colon vessels (black = colon ascendens (CA), light grey = colon transversum (CT), dark grey = colon descendens CD)) of the in vitro SHIME model measured in the headspace. The microbial suspension of the 3 vessels was incubated anaerobically for 24 hours with or without fresh feed medium or washed and resuspended in fresh feed medium. The supernatant of the SHIME suspension was incubated as well in the feed medium after filtersterilisation or autoclavation.