Induction of Tetraploids from Petiole Explants through Colchicine Treatments in Echinacea purpurea L.

Table 1

Comparison of the effect of colchicine concentration on shoot regeneration from petiole explants. Data were collected after 30 days on regeneration medium containing colchicine and 40 days on colchicine-free regeneration medium except for the control, data for which were collected after 40 days of culture without transfer to new medium.

Colchicine concentration (mg/L)	% Shoot regeneration	No. shoots per bottle
0 (control)	97.5 a*	16.2 a
30	67.5 b	6.2 b
60	50.0 c	5.0 c
120	20.0 d	2.0 c
240	7.5 e	0.8 d

*Data in the same column followed by different letters are significantly different by Duncan’t test at ≤5% level.