Modular Cre/lox System and Genetic Therapeutics for Colorectal Cancer

Figure 1

Schematic of the model system. Wnt signaling, which can be up- or downregulated by pharmacological or genetic modulators, promotes expression from Wnt-sensitive promoters, driving production of Cre recombinase. Cre excises stop cassettes from floxed expression vectors, allowing for production of effectors. Included in the effectors are genes that can positively or negatively feed back to influence Wnt activity and, hence, Cre expression. This model system can be generally applicable to any human disorder characterized by aberrant gene expression involving disorder-specific transcription factors, reproduced as per copyright agreement from [33].