BioMed Research International / 2009 / Article / Fig 2

Research Article

Herpesvirus-Mediated Delivery of a Genetically Encoded Fluorescent Sensor to Canine Cardiomyocytes

Figure 2

Representative low- and high-magnification transillumination of adult dog myocytes after isolation (day 0) and 1–3 days of culture (day 1, day 3). (a) shows the yield of the myocytes before and one day after culturing and infection. The high-magnification transillumination (b) shows the morphology of adult dog myocytes after isolation (day 0) and 1–3 day of culture (day 1, day 3) and the morphological changes of living cells, versus the culturing time from noninfected (right) and the virus-infected groups (left). After one day plated myocytes more than 80% displayed a rod-shaped morphology and healthy cross-striation. After 3 days (day 3) in culture, cells remained rod-shaped and partially cross-striated, and the main change was that cell ends became progressively more rounded.

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