Adding to Yersinia enterocolitica Gene Pool Diversity: Two Cryptic Plasmids from a Biotype 1A Isolate

Table 2

ORFs identified on plasmid pYe4449-2.

Name^1	Position (bp)	Strand	Size (aa)	MW^2 (kDa)	pI^2	Identities	Expect	Best BlastP match^3/function	Accession
ORF 1	446–913	+	155	16.8	5.3			hypothetical protein
ORF 2	957–3410	+	817	86.4	9.9			hypothetical protein
ORF 3 (yltA)	3933–4511	−	192	20.9	6.2			protein with unknown function expressed at low temperature
ORF 4	4511–5146	−	211	23.2	5.4			hypothetical protein
tnpR	5371–5997	+	208	23.3	9.6	92/208 (44%)	2e-46	Resolvase, N-terminal domain (Marinobacter aquaeolei VT8); TnpR-like protein	ref∣YP_957859.1∣
ORF 6	6107–6301	+	64	7.1	10.8			hypothetical protein
ORF 7 (ysrA)	6361–6744	+	127	13.5	4.2	42/113 (37%)	3e-10	hypothetical protein BACOVA_04330 (Bacteroides ovatus ATCC 8483); putative repeat protein	ref∣ZP_02067325.1∣
ORF8	6795–7478	−	227	25.3	5.3	52/127 (40%)	8e-18	hypothetical protein (Aeromonas hydrophila)	gb∣AAR06625.1∣
ORF 9	7643–8119	+	158	17.6	10.2			hypothetical protein
ORF 10	8637–9917	−	426	48.6	8	157/386 (40%)	2e-80	predicted P-loop ATPase (Bacteriophage APSE-3)	gb∣ACJ10118.1∣
ORF 11	10971–11462	+	163	18.2	6.1			hypothetical protein
ORF 12	11798–12250	-	150	16.5	5.8			hypothetical protein
ORF 13	12247–12534	−	95	9.9	4.5	72/95 (75%)	1e-28	hypothetical protein pKlebpneu12_p49 (Klebsiella pneumoniae)	ref∣YP_002286974.1∣
traC	12592–13803	−	403	44.8	6.3	345/401 (86%)	0	hypothetical protein pKlebpneu12_p48 (Klebsiella pneumoniae)	ref∣YP_002286973.1∣

¹Gene names according to putative conserved protein domains if available.²Molecular weights and isoelectric points are calculated using the algorithm from ExPASy’s Compute pI/Mw program.³Only significant BlastP matches with e-values below e-10 are listed; all others show e-values above 0.1 and are either indicated as “hypothetical protein” or experimental confirmation is specified.