Methodology Report

Efficient siRNA Delivery by the Cationic Liposome DOTAP in Human Hematopoietic Stem Cells Differentiating into Dendritic Cells

Figure 2

Cell viability during repeated, DOTAP-mediated transfection rounds in differentiating HSCs. Freshly isolated HSCs were induced to differentiate into immunogenic dendritic cells as described in the Materials and Methods. Immediately after treatment, cultures were split into two cell populations, of which one, used as the control, was grown in absence of manipulations (white bars), while the other was subjected to two and three DOTAP-based, scrambled siRNA transfection rounds performed at day 0 and day 3 (black bars), and day 0, day 3 and day 9 (grey bars). Cell viability was monitored by trypan blue staining at different time points as indicated.
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