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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 437284, 13 pages
http://dx.doi.org/10.1155/2009/437284
Research Article

Molecularly Characterised Xenograft Tumour Mouse Models: Valuable Tools for Evaluation of New Therapeutic Strategies for Secondary Liver Cancers

1Department of Pathobiology, Institute of Virology, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria
2Department of Data collection, Statistics, and Risk assessment, Austrian Agency for Health and Food Safety (AGES), Spargelfeldstrasse 191, 1226 Vienna, Austria
3Department of Biomedical Sciences, Institute of Animal Breeding and Genetics, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria
4VetOMICS, University of Veterinary Medicine, Veterinaerplatz 1, 1210 Vienna, Austria
5Department of Surgery, Medical University Vienna, Währinger Gürtel 18-20, 1090 Vienna, Austria
6Department of Pathology, Medical University of Graz, Auenbruggerplatz 25, 8036 Graz, Austria
7Division of Molecular and Experimental Surgery, Department of Surgery, University of Erlangen-Nürnberg, Schwabachanlage 10, 91054 Erlangen, Germany

Received 10 July 2008; Revised 5 November 2008; Accepted 19 December 2008

Academic Editor: Lisa Wiesmuller

Copyright © 2009 Daniela Mischek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To develop and evaluate new therapeutic strategies for the treatment of human cancers, well-characterised preclinical model systems are a prerequisite. To this aim, we have established xenotransplantation mouse models and corresponding cell cultures from surgically obtained secondary human liver tumours. Established xenograft tumours were patho- and immunohistologically characterised, and expression levels of cancer-relevant genes were quantified in paired original and xenograft tumours and the derivative cell cultures applying RT-PCR-based array technology. Most of the characteristic morphological and immunohistochemical features of the original tumours were shown to be maintained. No differences were found concerning expression of genes involved in cell cycle regulation and oncogenesis. Interestingly, cytokine and matrix metalloproteinase encoding genes appeared to be expressed differentially. Thus, the established models are closely reflecting pathohistological and molecular characteristics of the selected human tumours and may therefore provide useful tools for preclinical analyses of new antitumour strategies in vivo.