Research Article

Mesothelioma Cells Escape Heat Stress by Upregulating Hsp40/Hsp70 Expression via Mitogen-Activated Protein Kinases

Figure 5

Hsp40/70 rescue cells from heat stress. (a) Cells were pre-incubated with 5 or 10 nM of Hsp40 siRNA and then exposed to increasing heat stress (39–42°C) for 20 minutes. Following cells were kept under normal growth conditions for 48 hours and viable cells were counted after trypan blue exclusion staining. (b) Cells were pre-incubated with 5 or 10 nM of Hsp70 siRNA and treated as described above “a”. Data points represent the mean ± S.E.M. of triplicate experiments. * indicates compared to control (student’s paired -test, two-tailed). (c) Expression of caspase-3 by PDMM treated for various time intervals with increasing temperatures. Data points represent the mean ± S.E.M. of triplicate experiments.
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