Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
(a) Southern blot of Streptomyces sp. TN 58 BamHI-digested total DNA hybridised with 32P-labeled pSET152 plasmid. Lanes 1–8, the eight studied exconjugants; lane 9, untransformed wild type Streptomyces sp. TN 58; lane 10, BamHI-digested pSET152 plasmid DNA (5.7 Kb); lane 11, the 1 kb ladder used as DNA marker. (b) Schematic representation of the integration of plasmid pSET152 in the chromosome of Streptomyces sp. TN 58 by site-specific recombination.