Integrative Gene Cloning and Expression System for Streptomyces sp. US 24 and Streptomyces sp. TN 58 Bioactive Molecule Producing Strains
Natural variation of C31attB sites in various species of Streptomyces. (a) Alignment of attB nucleotide sequence of Streptomyces sp. US 24 strain (S. US 24) and Streptomyces sp. TN 58 (S. TN 58) with attB sequences of S. hygroscopicus NRRL5491 (S. hygro); S. avermitilis MA-4680 (S. aver); S. longisporoflavus 83E6 (S. longi); S. coelicolor (S. coel); S. clavugerus (S. clav); S. cinnamonensis (S. cinna); S. aureofaciens (S. aureo); S. lividans 66 TK64 (S. livid); S. griseus ATCC 12475 (S. grise); S. ambofaciens (S. ambof). Dark boxes, identical sequences; black shading sequence at which crossover occurs (TT) for the natural attB and attP sites. (b); Sequence of the attP site in C31.
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