Oxaprozin-Induced Apoptosis on CD40 Ligand-Treated Human Primary Monocytes Is Associated with the Modulation of Defined Intracellular Pathways
Figure 4
Oxaprozin-induced apoptosis of CD40L-treated monocytes involves the activation of caspase 3 . (a) Monocytes were cultured for 48 hours with control medium or 200 ng/mL CD40L plus 1 g/mL CD40L enhancer in the presence or absence of 100 M of oxaprozin. Then cells were lysed, and caspase 3 activity was determined by spectrophotometer. Data are expressed as mean SEM, n 3. (caspase 3 activity in absence versus in presence of CD40L: *P .05; caspase 3 activity in presence of CD40L versus CD40L plus oxaprozin: #P .05). (b) Monocytes were incubated for 48 hours with control medium or 200 ng/mL CD40L plus 1 g/mL CD40L enhancer in the presence or absence 100 M of oxaprozin and 50 M Ac-DEVD-CHO (caspase 3 inhibitor, CASP 3 INHIB). Data are expressed as mean SEM, n 3 (apoptosis in absence versus in presence of CD40L: *P .05; apoptosis in presence of CD40L versus CD40L plus oxaprozin: #P .05; apoptosis in presence of CD40L plus oxaprozin versus CD40L plus oxaprozin and caspase 3 inhibitor: §P .05).
