A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

Figure 3

Efficiency of different shRNA constructs in the inducing of silencing on Pokemon/GAPDH mRNA level as quantified by real-time RT-PCR. Total RNA was extracted from MCF-7 cells 36 hours after transfection with the indicated constructs. Normal MCF-7 cells were used as a negative control. An external standard curve was created to make sure that Pokemon and GAPDH were amplified at similar efficiency. Data shown represents, three replicated experiments with error bar indicated.