BioMed Research International / 2009 / Article / Fig 3

Research Article

A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

Figure 3

Efficiency of different shRNA constructs in the inducing of silencing on Pokemon/GAPDH mRNA level as quantified by real-time RT-PCR. Total RNA was extracted from MCF-7 cells 36 hours after transfection with the indicated constructs. Normal MCF-7 cells were used as a negative control. An external standard curve was created to make sure that Pokemon and GAPDH were amplified at similar efficiency. Data shown represents, three replicated experiments with error bar indicated.
514287.fig.003

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