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Journal of Biomedicine and Biotechnology
Volume 2009 (2009), Article ID 514287, 7 pages
http://dx.doi.org/10.1155/2009/514287
Research Article

A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

1The Key Laboratory of Chemical Biology, Division of Life Science, Graduate School at Shenzhen, Tsinghua University, Shenzhen, Guangdong 518055, China
2Department of Biological Science and Biotechnology, Tsinghua University, Beijing 100084, China
3Institute of Pharmacology, Medicine School, Tsinghua University, Beijing 100084, China

Received 19 November 2008; Revised 21 June 2009; Accepted 31 July 2009

Academic Editor: John McGregor

Copyright © 2009 Ma Weiwei et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. S. Guo and K. J. Kemphues, “par-1, a gene required for establishing polarity in C. Elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed,” Cell, vol. 81, no. 4, pp. 611–620, 1995. View at Google Scholar · View at Scopus
  2. M. Sohail, G. Doran, J. Riedemann, V. Macaulay, and E. M. Southern, “A simple and cost-effective method for producing small interfering RNAs with high efficacy,” Nucleic Acids Research, vol. 31, no. 7, p. e38, 2003. View at Google Scholar · View at Scopus
  3. O. Donzé and D. Picard, “RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase,” Nucleic Acids Research, vol. 30, no. 10, p. e46, 2002. View at Google Scholar · View at Scopus
  4. E. Bertrand, D. Castanotto, C. Zhou et al., “The expression cassette determines the functional activity of ribozymes in mammalian cells by controlling their intracellular localization,” RNA, vol. 3, no. 1, pp. 75–88, 1997. View at Google Scholar · View at Scopus
  5. X. G. Xia, H. Zhou, H. Ding, B. el Affar, Y. Shi, and Z. Xu, “An enhanced U6 promoter for synthesis of short hairpin RNA,” Nucleic Acids Research, vol. 31, no. 17, p. e100, 2003. View at Google Scholar · View at Scopus
  6. D. Boden, O. Pusch, F. Lee, L. Tucker, P. R. Shank, and B. Ramratnam, “Promoter choice affects the potency of HIV-1 specific RNA interference,” Nucleic Acids Research, vol. 31, no. 17, pp. 5033–5038, 2003. View at Publisher · View at Google Scholar · View at Scopus
  7. J.-Y. Yu, S. L. DeRuiter, and D. L. Turner, “RNA interference by expression of short-interfering RNAs and hairpin RNAs in mammalian cells,” Proceedings of the National Academy of Sciences of the United States of America, vol. 99, no. 9, pp. 6047–6052, 2002. View at Publisher · View at Google Scholar · View at Scopus
  8. P. J. Paddison, A. A. Caudy, E. Bernstein, G. J. Hannon, and D. S. Conklin, “Short hairpin RNAs (shRNAs) induce sequence-specific silencing in mammalian cells,” Genes and Development, vol. 16, no. 8, pp. 948–958, 2002. View at Publisher · View at Google Scholar · View at Scopus
  9. F. Czauderna, A. Santel, M. Hinz et al., “Inducible shRNA expression for application in a prostate cancer mouse model,” Nucleic Acids Research, vol. 31, no. 21, p. e127, 2003. View at Google Scholar · View at Scopus
  10. Y.-D. Kwak, H. Koike, and K. Sugaya, “RNA interference with small hairpin RNAs transcribed from a human U6 promoter-driven DNA vector,” Journal of Pharmacological Sciences, vol. 93, no. 2, pp. 214–217, 2003. View at Publisher · View at Google Scholar · View at Scopus
  11. G. Tiscornia, V. Tergaonkar, F. Galimi, and I. M. Verma, “CRE recombinase-inducible RNA interference mediated by lentiviral vectors,” Proceedings of the National Academy of Sciences of the United States of America, vol. 101, no. 19, pp. 7347–7351, 2004. View at Publisher · View at Google Scholar · View at Scopus
  12. X. Coumoul, V. Shukla, C. Li, R. H. Wang, and C. X. Deng, “Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference,” Nucleic Acids Research, vol. 33, no. 11, p. e102, 2005. View at Google Scholar · View at Scopus
  13. M. Li and B. Rohrer, “Gene silencing in Xenopus laevis by DNA vector-based RNA interference and transgenesis,” Cell Research, vol. 16, no. 1, pp. 99–105, 2006. View at Publisher · View at Google Scholar · View at Scopus
  14. T. R. Brummelkamp, R. Bernards, and R. Agami, “A system for stable expression of short interfering RNAs in mammalian cells,” Science, vol. 296, no. 5567, pp. 550–553, 2002. View at Publisher · View at Google Scholar · View at Scopus
  15. J. Seibler, B. Küter-Luks, H. Kern et al., “Single copy shRNA configuration for ubiquitous gene knockdown in mice,” Nucleic Acids Research, vol. 33, no. 7, p. e67, 2005. View at Google Scholar · View at Scopus
  16. S. Pebernard and R. D. Iggo, “Determinants of interferon-stimulated gene induction by RNAi vectors,” Differentiation, vol. 72, no. 2-3, pp. 103–111, 2004. View at Publisher · View at Google Scholar · View at Scopus
  17. H. Kawasaki and K. Taira, “Short hairpin type of dsRNAs that are controlled by tRNA(Val) promoter significantly induce RNAi-mediated gene silencing in the cytoplasm of human cells,” Nucleic Acids Research, vol. 31, no. 2, pp. 700–707, 2003. View at Publisher · View at Google Scholar · View at Scopus
  18. K. Oshima, H. Kawasaki, Y. Soda, K. Tani, S. Asano, and K. Taira, “Maxizymes and small hairpin-type RNAs that are driven by a tRNA promoter specifically cleave a chimeric gene associated with leukemia in vitro and in vivo,” Cancer Research, vol. 63, no. 20, pp. 6809–6814, 2003. View at Google Scholar · View at Scopus
  19. M. S. Brenz Verca, P. Weber, C. Mayer et al., “Development of a species-specific RNA polymerase I-based shRNA expression vector,” Nucleic Acids Research, vol. 35, no. 2, p. e10, 2007. View at Publisher · View at Google Scholar · View at Scopus
  20. J. Su, Z. Zhu, F. Xiong, and Y. Wang, “Hybrid cytomegalovirus-U6 promoter-based plasmid vectors improve efficiency of RNA interference in zebrafish,” Marine Biotechnology, vol. 10, no. 5, pp. 511–517, 2008. View at Publisher · View at Google Scholar · View at Scopus
  21. J. A. Sawicki, R. J. Morris, B. Monks, K. Sakai, and J.-I. Miyazaki, “A composite CMV-IE enhancer/beta-actin promoter is ubiquitously expressed in mouse cutaneous epithelium,” Experimental Cell Research, vol. 244, no. 1, pp. 367–369, 1998. View at Publisher · View at Google Scholar · View at Scopus
  22. N. S. Yew, D. M. Wysokenski, K. X. Wang et al., “Optimization of plasmid vectors for high-level expression in lung epithelial cells,” Human Gene Therapy, vol. 8, no. 5, pp. 575–584, 1997. View at Google Scholar · View at Scopus
  23. T. Maeda, R. H. Hobbs, T. Merghoub et al., “Role of the proto-oncogene Pokemon in cellular transformation and ARF repression,” Nature, vol. 433, no. 7023, pp. 278–285, 2005. View at Publisher · View at Google Scholar · View at Scopus
  24. T. Maeda, R. M. Hobbs, and P. P. Pandolfi, “The transcription factor Pokemon: a new key player in cancer pathogenesis,” Cancer Research, vol. 65, no. 19, pp. 8575–8578, 2005. View at Publisher · View at Google Scholar · View at Scopus
  25. J.-P. Lai, W. Z. Ho, L. E. Kilpatrick et al., “Full-length and truncated neurokinin-1 receptor expression and function during monocyte/macrophage differentiation,” Proceedings of the National Academy of Sciences of the United States of America, vol. 103, no. 20, pp. 7771–7776, 2006. View at Publisher · View at Google Scholar · View at Scopus
  26. P. Hatzis and I. Talianidis, “Dynamics of enhancer-promoter communication during differentiation-induced gene activation,” Molecular Cell, vol. 10, no. 6, pp. 1467–1477, 2002. View at Publisher · View at Google Scholar · View at Scopus
  27. P. Hatzis, I. Kyrmizi, and I. Talianidis, “Mitogen-activated protein kinase-mediated disruption of enhancer-promoter communication inhibits hepatocyte nuclear factor 4alpha expression,” Molecular and Cellular Biology, vol. 26, no. 19, pp. 7017–7029, 2006. View at Publisher · View at Google Scholar · View at Scopus