BioMed Research International / 2009 / Article / Tab 1

Research Article

A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

Table 1

Sequences of oilgonucleotides used in the research.

shRNASequences of oilgonucleotidesTarget site on pokemon mRNA (GenBank accession number NM_01 5898)

1F1: -ccgtgtacgagatcgacttcttcaagagagaagtcgatctcgtacacgtttttt- 383–401 nt
   R1: -ccggggcacatgctctagctgaagaagttctctcttcagctagagcatgtgcaaaaaatcga-
2F2: -ccggcttagacttctatgggttcaagagacccatagaagtctaagccgtttttt- 809–827 nt
   R2: -ccggggccgaatctgaagatacccaagttctctgggtatcttcagattcggcaaaaaatcga-
3F3: -cgggcgtcatggactactacttcaagagagtagtagtccatgacgccctttttt- 1202–1200 nt
   R3: -ccgggcccgcagtacctgatgatgaagttctctcatcatcaggtactgcgggaaaaaatcga-

shRNA encoding cassettes 1–3 were formed by annealing two corresponding oligonucleotides, respectively. F stands for forward and R for reverse.

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