A Significant Increase of RNAi Efficiency in Human Cells by the CMV Enhancer with a tRNAlys Promoter

Table 1

Sequences of oilgonucleotides used in the research.

shRNA	Sequences of oilgonucleotides	Target site on pokemon mRNA (GenBank accession number NM_01 5898)
1	F1:  5^′-ccgtgtacgagatcgacttcttcaagagagaagtcgatctcgtacacgtttttt-3^′	383–401 nt
	R1:  3^′-ccggggcacatgctctagctgaagaagttctctcttcagctagagcatgtgcaaaaaatcga-5^′
2	F2:  5^′-ccggcttagacttctatgggttcaagagacccatagaagtctaagccgtttttt-3^′	809–827 nt
	R2:  3^′-ccggggccgaatctgaagatacccaagttctctgggtatcttcagattcggcaaaaaatcga-5^′
3	F3:  5^′-cgggcgtcatggactactacttcaagagagtagtagtccatgacgccctttttt-3^′	1202–1200 nt
	R3:  3^′-ccgggcccgcagtacctgatgatgaagttctctcatcatcaggtactgcgggaaaaaatcga-5^′

shRNA encoding cassettes 1–3 were formed by annealing two corresponding oligonucleotides, respectively. F stands for forward and R for reverse.