High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas
Figure 2
ROC curve analysis of Quantitative Methylation-specific PCR results. (a) ROC curve for QMSP assay was designed on the basis of MGMT/ACTB ratios in determined in normal brain tissues () and total tumour samples (). The area under the curve (AUC) is 0.74 (). (b) Comparison of ROC curves were designed on the basis of MGMT/ACTB ratios determined in normal brain tissues () and FFPE () or snap-frozen specimens (). The area under the curve (AUC) is 0.70 () for FFPE samples and 0.79 () for snap-frozen specimens. (c) Scatter plot in logarithm scale of MGMT/ACTB ratios in normal brain tissue (NBT), tumour FFPE samples, and snap-frozen (SnF) specimens. *indicates samples with an MGMT/ACTB ratio of 0 which cannot be plotted on a logarithm scale ( for NBT, for FFPE and for SnF). The horizontal line indicates the optimal cutoff value of 0.35.
