BioMed Research International / 2009 / Article / Fig 2

Research Article

High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas

Figure 2

ROC curve analysis of Quantitative Methylation-specific PCR results. (a) ROC curve for QMSP assay was designed on the basis of MGMT/ACTB ratios in determined in normal brain tissues ( ) and total tumour samples ( ). The area under the curve (AUC) is 0.74 ( ). (b) Comparison of ROC curves were designed on the basis of MGMT/ACTB ratios determined in normal brain tissues ( ) and FFPE ( ) or snap-frozen specimens ( ). The area under the curve (AUC) is 0.70 ( ) for FFPE samples and 0.79 ( ) for snap-frozen specimens. (c) Scatter plot in logarithm scale of MGMT/ACTB ratios in normal brain tissue (NBT), tumour FFPE samples, and snap-frozen (SnF) specimens. *indicates samples with an MGMT/ACTB ratio of 0 which cannot be plotted on a logarithm scale ( for NBT, for FFPE and for SnF). The horizontal line indicates the optimal cutoff value of 0.35.
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531692.fig.002b
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