High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas

Table 1

MGMT methylation frequencies in normal brain tissues and glioma samples.

MGMT * status	Normal brain tissues 𝑛=28		FFPE samples^#   𝑛=28		Frozen samples 𝑛=22		Total tumour samples 𝑛=50
	QMSP	MSP	QMSP	MSP	QMSP	MSP	QMSP	MSP
neg	26 (92.9%)	18 (64.3%)	13 (46.4%)	15 (53.6%)	8 (36.4%)	7 (31.8%)	21 (42.0%)	22 (44.0%)
pos	2 (7.1%)	10 (35.7%)	15 (53.6%)	13 (46.4%)	14 (63.6%)	15 (68.2%)	29 (58.0%)	28 (56.0%)

*neg: Methylation levels for QMSP are below the cutoff point of 0.35, no band is seen in gel electrophoresis for MSP; pos: Methylation levels for QMSP exceed the cutoff point of 0.35, a band is seen in gel electrophoresis for MSP. ^#FFPE: Formalin-fixed paraffin-embedded.