High Specificity of Quantitative Methylation-Specific PCR Analysis for MGMT Promoter Hypermethylation Detection in Gliomas

Table 3

Comparison of MGMT methylation status as determined by Quantitative Methylation-Specific PCR (QMSP) and. Methylation-Specific PCR (MSP).

		NBT*𝑛=28		FFPE*𝑛=28		SF*𝑛=22		Tumours*𝑛=50		Total samples*𝑛=78
		MSP +	MSP-	MSP +	MSP-	MSP +	MSP-	MSP +	MSP-	MSP +	MSP-
QMSP	<0.35	8	18	0	13	3	5	3	18	11	36
	>0.35	2	0	13	2	12	2	25	4	27	4
𝜅^#		0.243		0.860		0.495		0.715		0.613
95%CI		0.084–0.40		0.73–0.99		0.286–0.704		0.59–0.84		0.505–0.712
𝑃		.049		𝑃<.0001		𝑃=.00195		<.0001		<.0001

*NBT: Normal brain tissue; FFPE: Formalin fixed paraffin embedded specimens, SF: Snap-frozen specimen; MSP +: A band is seen in gel electrophoresis; -: No band is seen in gel electrophoresis. ^#𝜅:  Measure of the degree of concordance between MSP and QMSP (see Materials and Methods), 95%CI, Confidence interval.