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Journal of Biomedicine and Biotechnology
Volume 2009, Article ID 562943, 7 pages
Research Article

Effect of Plant Oils upon Lipase and Citric Acid Production in Yarrowia lipolytica Yeast

1Department of Biology, Faculty of Science, University of Isfahan, Isfahan 81746-73696, Iran
2Department of Chemistry, Faculty of Science, University of Isfahan, Isfahan 81746-73441, Iran

Received 2 May 2009; Accepted 20 July 2009

Academic Editor: Isabel Sá-Correia

Copyright © 2009 Farshad Darvishi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The nonconventional yeast Yarrowia lipolytica degrades very efficiently hydrophobic substrates to produce organic acids, single-cell oil, lipases, and so forth. The aim of this study was to investigate the biochemical behavior and simultaneous production of valuable metabolites such as lipase, citric acid (CA), and single-cell protein (SCP) by Yarrowia lipolytica DSM 3286 grown on various plant oils as sole carbon source. Among tested plant oils, olive oil proved to be the best medium for lipase and CA production. The Y. lipolytica DSM 3286 produced 34.6 ± 0.1 U/mL of lipase and also CA and SCP as by-product on olive oil medium supplemented with yeast extract. Urea, as organic nitrogen, was the best nitrogen source for CA production. The results of this study suggest that the two biotechnologically valuable products, lipase and CA, could be produced simultaneously by this strain using renewable low-cost substrates such as plant oils in one procedure.