DNA, RNA, and Protein Extraction: The Past and The Present

Table 1

Typical biological interactions used in affinity chromatography [42].

Types of ligand	Target molecules
Enzyme	Substrate analogue, inhibitor, cofactor
Antibody	Antigen, virus, cell
Lectin	Polysaccharide, glycoprotein, cell surface receptor, cell
Nucleic acid	Complementary base sequence, histones, nucleic acid polymerase, nucleic acid binding protein
Hormone, vitamin	Receptor, carrier protein
Glutathione	Glutathione-S-transferase or GST fusion proteins
Metal ions	Poly (his) fusion proteins, native proteins with histidine, cysteine and/ortryptophan residues on their surfaces