A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag
Figure 2
SDS-PAGE analysis of the expression and purification of sfGFP-TEV-His6 Nd1–6 and TEV-His6. (a) Lanes 1–3 are the result for TEV-His6: Lane 1: autoinduced whole-bacterial lysate; Lane 2: flow through from Ni-NTA affinity chromatography; Lane 3: the purified TEV-His6 eluted from Ni-NTA affinity chromatography. Lanes 4–7 are the result for sfGFP-TEV-His6 Nd2: Lane 4: autoinduced whole-bacterial lysate; Lane 5: flow through from Ni-NTA affinity chromatography; Lane 6: the purified sfGFP-TEV-His6 Nd2 eluted from Ni-NTA affinity chromatography; Lane 7: the pooled sample of purified sfGFP-TEV-His6 Nd2 eluted from Q anion exchange chromatography. (b) The results for sfGFP-TEV-His6 Nd1 and Nd3–6. “Ni” represents the results of the purified protein eluted from Ni-NTA affinity chromatography; “Q” represents the pooled sample of purified protein eluted from Q anion exchange chromatography. “M” in (a) and (b) represents the protein marker.
