A Novel Method for High-Level Production of TEV Protease by Superfolder GFP Tag

Table 3

Purification results of sfGFP-TEV-His₆ Nd1–6 and TEV-His₆ collected from 1-L of expression culture medium.

Abbreviation	Theoretical MW (kDa)	Total protein after Ni-NTA (mg)	Purity	Total protein after High Q (mg)	Purity	Total activity (𝜇mol/h)	Specific activity (𝜇mol/h/mg)
Nd1	53.5	244±1	90%	221±2	96%	7.3±0.2	0.067±0.002
Nd2	53.8	334±2	94%	323±1	98%	13.7±0.3	0.085±0.002
Nd3	54.1	220±4	93%	211±3	97%	8.0±0.1	0.076±0.001
Nd4	54.5	304±6	93%	293±4	98%	11.9±0.5	0.082±0.003
Nd5	55.0	299±1	93%	288±2	98%	11.3±0.6	0.079±0.005
Nd6	54.2	308±5	93%	300±4	96%	12.0±0.5	0.080±0.003
TEV	28.8	140±3	98%	—	—	12.6±0.6	0.090±0.005