Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein
Construction of the pIE1/153A (V4) expression plasmid carrying the hIL-7 gene. The
hIL-7 gene from pORF9-hIL07 vector was inserted in pIE1/153A(V4) expression plasmid,
pIE1/153A(V4)/hIL-7 and pBmA-pac were cotransfected into Sf9 cells. Clones producing hIL-7
were selected using puromycin and analyzed by western blot and SDS page.