BioMed Research International / 2009 / Article / Fig 1

Research Article

Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

Figure 1

Construction of the pIE1/153A (V4) expression plasmid carrying the hIL-7 gene. The hIL-7 gene from pORF9-hIL07 vector was inserted in pIE1/153A(V4) expression plasmid, pIE1/153A(V4)/hIL-7 and pBmA-pac were cotransfected into Sf9 cells. Clones producing hIL-7 were selected using puromycin and analyzed by western blot and SDS page.