BioMed Research International / 2009 / Article / Fig 2

Research Article

Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

Figure 2

Screening of puromycin resistant clones in Sf9 cells co-transfected with pIE1/153A (V4) expression plasmid carrying hIL-7 gene and pBmA-pac carrying puromycin resistance gene. Cells were placed in medium containing puromycin at a final concentration of 5  gmL−1 for 3 weeks. Lane 1: protein marker. Lanes 2–11: polyclones Sf9hIL-7G, Sf9hIL-7E, Sf9hIL-7F, Sf9hIL-7A1, Sf9hIL-7B4, Sf9hIL-7D5, Sf9hIL-7H, Sf9hIL-7C1-2, Sf9hIL-7C1-3, and Sf9hIL-B4-1, respectively.