BioMed Research International / 2009 / Article / Fig 5

Research Article

Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein

Figure 5

Production profile of hIL-7 in stably transfected Sf9 with two different expressions, cells were grown in serum-free medium (Sf900-II) in shaker flasks. The amount of hIL-7 in supernatants was measured by sandwich ELISA using rabbit antihuman IL-7 antibody as the capture antibody and a monoclonal antihuman IL-7 as the detection antibody. Streptavidin-horseradish peroxidase was used for conversion of a substrate mixture containing stabilized hydrogen peroxide, and stabilized tetramethylbenzidine commercially available as purified human IL-7 was used as a standard ( ).