Nonviral Production of Human Interleukin-7 in Spodoptera Frugiperda Insect Cells as a Soluble Recombinant Protein
Biological activity assay of recombinant human IL-7 produced in insect cells.
IL-7-dependent murine immature B lymphocyte cell line 2E8 was used for proliferation assay. After 72 hours
incubation at 37°C in a 5% C atmosphere, cell proliferation was detected by
tetrazolium compound MTS and an electron coupling reagent PMS. The absorbance of the formazan
was measured at 490 nm. Commercially available rhIL-7 was used to establish the 2E8 bioassay.
Medium alone served as a negative control, and each sample was assayed in triplicate ().