An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum
Genetic organization of a recombinant construct containing a defined deletion. The map shows the recombinant (1488 bp) region deleted from the SP genome. The open arrow indicates the coding region of the asd gene ( gene), and dotted lines represent the limits of the deleted region. The deletion is shown as an open triangle, and the sizes of the flanking regions adjacent to the deletion on the suicide vector are indicated. The position and orientation of PCR primers used in this study are indicated by filled arrows on the map of the wt DNA. The sizes of the PCR amplified products from the wt, the SP (Cm) mutant, and the SP mutant are 1796 bp, 1360 bp, and 328 bp, respectively.
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