BioMed Research International / 2009 / Article / Fig 3

Methodology Report

An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Figure 3

Genetic organization of a recombinant construct containing a defined deletion. The map shows the recombinant (1488 bp) region deleted from the  SP genome. The open arrow indicates the coding region of the asd  gene ( gene), and dotted lines represent the limits of the deleted region. The deletion is shown as an open triangle, and the sizes of the flanking regions adjacent to the deletion on the suicide vector are indicated. The position and orientation of PCR primers used in this study are indicated by filled arrows on the map of the wt DNA. The sizes of the PCR amplified products from the wt, the  SP      (Cm)   mutant, and the  SP   mutant are 1796 bp, 1360 bp, and 328 bp, respectively.
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