An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Table 1

Strains and plasmids used in this study.

	Name	Description	Source	Reference
Strains	S. Pullorum S06004 (𝑁𝑎𝑙^R)	Recipient	Lab collection
	𝜒7213 (pGMB151-Δ𝑎𝑠𝑑/Cm)	Donor	This work
	𝜒7213 (𝐾𝑚^RΔ𝑎𝑠𝑑)	E. coli for cloning pGMB151	Dr. Roy Curtiss III	Kang et al. [5]
	Spy372 (𝜆-pir)	E. coli for cloning pGMB151	Lab collection	Huang et al. [9]
	E. coli. DH5𝛼	E. coli for cloning pMD18	Lab collection
	pKD3	Plasmid containing 𝐶𝑚^R gene	Dr. Cristina Marolda	Datsenko and Wanner [10]
Plasmids	pCP20	Plasmid of expressing FLP	Dr. Cristina Marolda	Datsenko and Wanner [10]
	pGMB151 (𝐴𝑚𝑝^R 𝑆𝑚^R)	Suicide plasmid	Lab collection	Huang et al. [9]
	pYA3334 (with asd  gene)	Plasmid of expressing DAP	Dr. Roy Curtiss III	Kang et al. [8]
	pYA3334-dsRED	Plasmid of expressing dsRED	This work
	pMD18	Plasmid for cloning	Takara
	pMD-asdp12	asdp12 cloned into pMD18	This work
	pMD-asdp34	asdp34 cloned into pMD18	This work
	pMD-𝐶𝑚^R	𝐶𝑚^R gene cloned into pMD18	This work
	pMD-asdp1234	asdp1234 cloned into pMD18	This work
	pMD-asdp1234(Cm)	asdp1234(Cm) cloned into pMD18	This work