BioMed Research International / 2009 / Article / Tab 1

Methodology Report

An Improved Method to Knock Out the asd Gene of Salmonella enterica Serovar Pullorum

Table 1

Strains and plasmids used in this study.

Name Description Source Reference

Strains S. Pullorum S06004 ( )RecipientLab collection
(pGMB151- Cm)DonorThis work
( )E. coli for cloning pGMB151Dr. Roy Curtiss IIIKang et al. [5]
Spy372 ( -pir)E. coli for cloning pGMB151Lab collectionHuang et al. [9]
E. coli. DH5 E. coli for cloning pMD18Lab collection
pKD3Plasmid containing geneDr. Cristina MaroldaDatsenko and Wanner [10]

PlasmidspCP20Plasmid of expressing FLPDr. Cristina MaroldaDatsenko and Wanner [10]
pGMB151 ( )Suicide plasmidLab collectionHuang et al. [9]
pYA3334 (with asd  gene)Plasmid of expressing DAPDr. Roy Curtiss IIIKang et al. [8]
pYA3334-dsREDPlasmid of expressing dsREDThis work
pMD18 Plasmid for cloningTakara
pMD-asdp12asdp12 cloned into pMD18This work
pMD-asdp34asdp34 cloned into pMD18This work
pMD- gene cloned into pMD18This work
pMD-asdp1234asdp1234 cloned into pMD18This work
pMD-asdp1234(Cm)asdp1234(Cm) cloned into pMD18This work

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