Quantification of Small Non-Coding RNAs Allows an Accurate Comparison of miRNA Expression Profiles

Table 2

Real-time PCR data obtained for HeLa and LCL were compared calculating the ΔCt differences between Ct (10 ng of total RNA) and Ct (10 ng of LMW RNA) values for miR-21 and snoZ30. Standard deviations of at least three independent assays are reported in parentheses. LMW RNA concentrations (%) calculated using the formula 2^−ΔCt·100 are also reported where ΔCt are referred to snoZ30 values. Standard deviations of at least three independent assays are reported in parentheses.

		ΔCt (total RNA-LMW RNA)		Calculated LMW RNA (%) (2^−ΔCt(snoZ30)⋅100)
Cell Type	Extraction Method	miR-21	snoZ30
HeLa	TRIzol reagent	1.8 (2)	2.1 (1)	23 (±2)
	MirVana kit	2.5 (1)	2.9 (1)	13 (±1)
	RNEasy kit	3.5 (4)	5.1 (3)	3 (±0.5)
LCL	TRIzol reagent	2.3 (1)	2.4 (1)	19 (±1)
	MirVana kit	2.2 (1)	2.1 (4)	23 (±5)
	RNEasy kit	4.2 (2)	4.6 (4)	4 (±1)