BioMed Research International / 2009 / Article / Fig 1

Research Article

Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

Figure 1

Western blots performed with antibodies, DAb1(a), 1.9A(b), 4.8E(c), and 3.9G(d). The four different clones each recognises the recombinant -Hb, giving a correct band of 17 kDa ( -Hb protein fused with His-Tag; adding 8 amino acids). Furthermore they all recognise a slightly smaller band of 146 amino acid residues in the K562 extract, containing the -Hb. A difference is observed for the recognised band in the lane containing the recombinant -Hb and the -Hb in the K562 extract. Lane H: a control his-tagged protein, R: adult red blood cell extract, K: K562 cell extract, and E: recombinant -Hb chain (2,5  g). (e) Representative ponceau staining of the blotted proteins. M: the marker.

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