Epsilon Haemoglobin Specific Antibodies with Applications in Noninvasive Prenatal Diagnosis

Figure 1

Western blots performed with antibodies, DAb1(a), 1.9A(b), 4.8E(c), and 3.9G(d). The four different clones each recognises the recombinant 𝜀-Hb, giving a correct band of 17 kDa (𝜀-Hb protein fused with His-Tag; adding 8 amino acids). Furthermore they all recognise a slightly smaller band of 146 amino acid residues in the K562 extract, containing the 𝜀-Hb. A difference is observed for the recognised band in the lane containing the recombinant 𝜀-Hb and the 𝜀-Hb in the K562 extract. Lane H: a control his-tagged protein, R: adult red blood cell extract, K: K562 cell extract, and E: recombinant 𝜀-Hb chain (2,5 𝜇g). (e) Representative ponceau staining of the blotted proteins. M: the marker.